Peptide Arrays on Membrane Supports

Peptide Arrays on Membrane Supports

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Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.The majority of mammalian species express five different classes of antibodies ( immunoglobulins): IgG, IgM, IgA, Ig|D, and IgE. ... fur Biochemie, Biozentrum N210/20, Marie-Curie-Straao┬×e 9, 60439 Frankfurt am Main, Germany M. Mahler, Institut fur Molekulare Genetik, ... 79111 Freiburg, Germany M. BlA¼thner Institut fur Molekulare Genetik, UniversitAct Heidelberg, Germany Springer Lab Manual J. Koch, anbsp;...

Title:Peptide Arrays on Membrane Supports
Author: Joachim Koch, Michael Mahler
Publisher:Springer Science & Business Media - 2002-02-28

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